Journal: Molecular Cytogenetics

Article Title: Noninvasive prenatal diagnosis of fetal aneuploidy by circulating fetal nucleated red blood cells and extravillous trophoblasts using silicon-based nanostructured microfluidics

doi: 10.1186/s13039-017-0343-3

Figure Lengend Snippet: Array comparative genomic hybridization (aCGH) and next generation sequencing (NGS) for the same whole genome amplification (WGA) products of captured fnRBC from 5 pregnant women. WGA product from Promega male DNA (Promega, Wisconsin, USA) was used as reference. The aCGH were performed with GenetiSure Pre-Screen Array Kit 8x60K (Agilent Technologies, CA, USA) on a G4900DA SureScan microarray scanner (Agilent Technologies). NGS was performed using Ion PGM Hi-Q Sequencing Kit with Ion 316 chip (Thermo Fisher Scientific, California, USA) on the Ion Torrent PGM Instrument (Thermo Fisher Scientific) platform. Aneuploidy chromosomes are indicated by arrows. Aneuploidy chromosomes are indicated by arrows. The results of aCGH are comparable with that of NGS

Article Snippet: The aCGH were performed with GenetiSure Pre-Screen Array Kit 8x60K (Agilent Technologies, CA, USA) on a G4900DA SureScan microarray scanner (Agilent Technologies).

Techniques: Hybridization, Next-Generation Sequencing, Whole Genome Amplification, Microarray, Sequencing

Journal: Molecular Cytogenetics

Article Title: A pilot proof-of-principle study to compare fresh and vitrified cycle preimplantation genetic screening by chromosome microarray and next generation sequencing

doi: 10.1186/s13039-016-0238-8

Figure Lengend Snippet: Exemplified PGS results by use of rapid aCGH (DNA hybridization for 2 h), regular aCGH (DNA hybridization for 16 h) and next generation sequencing (NGS) for the same WGA products. Rapid and regular aCGH were performed with CytoScan 60 K microarray chip (Agilent customer array, Changhua Christian Hospital, Taiwan) on a G4900DA SureScan microarray scanner (Agilent Technologies, CA, USA). NGS was performed using Ion PGM Hi-Q Sequencing Kit with Ion 316 chip (Life technologies, California, USA) on the Ion Torrent PGM Instrument (Life technologies) platform. Aneuploidy chromosomes or chromosomal fragments are indicated by arrows. Some atypical segmental gains and/or losses with copy number change < 1 but > 0.5 (a likely result of embryo mosaicism) were also classified as segmental aneuploidies and marked by stars. The results of rapid aCGH are comparable with that of regular aCGH and NGS

Article Snippet: Rapid and regular aCGH were performed with CytoScan 60 K microarray chip (Agilent customer array, Changhua Christian Hospital, Taiwan) on a G4900DA SureScan microarray scanner (Agilent Technologies, CA, USA).

Techniques: DNA Hybridization, Next-Generation Sequencing, Microarray, Sequencing